Molecular identification of pathogenic fungi.

Identification of Mucorales isolates from soil using morphological and molecular methods

Background and Purpose: Soil is the main habitat of saprophytic and pathogenic fungi. Mucormycetes are one of the most parts of soil fungi and certain members are among opportunistic fungi and can cause systemic fungal infections in immunocompromised patients. The majority of human and animal infections are caused by members of the genera Rhizopus, Mucor, Rhizomucor, Lichtheimia (Absidia), Cunn...

Molecular identification of non-Cryptococcus yeasts associated with pigeon droppings in Shiraz, Southern Iran

Background: Birds are considered as a reservoir for pathogenic and non-pathogenic fungi. Pigeon droppings have the potential for spreading these fungi to the environment. Cryptococcus species are important fungi associated with pigeon droppings. In this regard, there are many types of yeast associated with guano that is important for human and animal health. Ai...

Rolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species

Conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. These tests often need several days or weeks to complete and are frequentlyunspecific. Molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. The Rolling CircleAmplification app...

Studies of human pathogenic fungi: development of identification schemes, use of molecular genetic methods for their detection and evaluation of their susceptibilities

Development and evaluation of nucleic acid-based techniques for an auxiliary diagnosis of invasive fungal infections in formalin-fixed and paraffin-embedded (FFPE) tissues.

Notwithstanding the advantages of providing definitive diagnoses, the identification of fungi based on histopathological determination can be difficult and may lead to a pit of diagnoses. Therefore, the establishment of an auxiliary diagnostic method for use in routine pathological laboratories is desirable and should improve the above situation. Our previous studies have shown the superiority ...

Identification of some fungi accompanying the scab symptoms in Iran

Some fungal species were isolated from scab or scab-like symptoms on leaves of various plant hosts in Iran. Some of them isolated from apple and pear leaves were investigated in the present study. The isolates were identified based on the morphological and cultural characteristics. On the other hand, for molecular identification and phylogenetic analyses were carried out based on the sequence o...